SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF CEPHRADINE OR CEFTAZIDINE IN HUMAN URINE USING BATCH AND FLOW-INJECTION PROCEDURES

Sensitive and fast spectrophotometric methods for the determination of cephradine or ceftazidine in human urine, based on the formation of c ompounds between these drugs and Pd(II), are described. In the batch p rocedures the calibration graphs resulting from the measurement of the absorbance at 330 nm is linear over the range 5.0-60.0 mu g ml(-1) fo r cephradine and 3.0-60.0 mu g ml(-1) for ceftazidine. The methods wer e successfully adapted to FI-systems, the peak heights being proportio nal to cephalosporin concentration over the range 5.0-60.0 mu g ml(-1) for cephradine and 3.0-60.0 mu g ml(-1) for ceftazidine. The sampling frequency was 60 h(-1) with a sample injection of 72 mu l. (C) 1997 P ublished by Elsevier Science B.V.