Cycloprodigiosin hydrochloride, a H+/Cl- symporter, induces apoptosis in human colon cancer cell lines in vitro

Recently, rye reported that cycloprodigiosin hydrochloride (cPrG . HCl), a novel H+/Cl- symporter, induces acidification of the cytosol and lends to a poptosis on rat and human liver cancer cells. In the present study, the eff ects of cPrG . HCl, a H+/Cl- symporter, were examined in colon cancer cell lines in vitro. In the MTT assay, cPrG . HCl inhibited the growth of two co lon cancer cell lines (WiDr and SW480) in a dose- and time-dependent manner . The cPrG . HCl treatment of both types of cells induced apoptosis as conf irmed by the appearance of a sub-G(1) population and intranucleosomal DNA f ragmentation. In addition, cPrG . HCl lowered pHi (below pH 6.8) respective ly. Therefore, these results suggest that cPrG . HCl may be useful for the treatment of colon cancer cells.