ENHANCED MITOTIC DIVISION OF CULTURED PASSIFLORA AND PETUNIA PROTOPLASTS BY OXYGENATED PERFLUOROCARBON AND HEMOGLOBIN

Authors
ANTHONY P DAVEY MR POWER JB LOWE KC
Citation
P. Anthony et al., ENHANCED MITOTIC DIVISION OF CULTURED PASSIFLORA AND PETUNIA PROTOPLASTS BY OXYGENATED PERFLUOROCARBON AND HEMOGLOBIN, Biotechnology techniques, 11(8), 1997, pp. 581-584
Citations number
15
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
8
Year of publication
1997
Pages
581 - 584
Database
ISI
SICI code
0951-208X(1997)11:8<581:EMDOCP>2.0.ZU;2-M
Abstract
Cell suspension-derived protoplasts were cultured in (A) liquid medium , (B) medium overlaying oxygenated perfluorodecalin (PFC), (C) medium containing 1:50 (v:v) of haemoglobin solution (Erythrogen(TM)), or (D) medium with 1:50 (v:v) Erythrogen(TM) overlaying oxygenated PFC. In P assiflora, mitotic division of protoplasts was increased (P < 0.05) by all treatments, with Erythrogen(TM) being the most effective (120% in crease over control). For Petunia, treatment D induced maximum mitosis (140% over control), whilst Erythrogen(TM) alone produced a less pron ounced (80% over control) increase.