INVESTIGATIONS OF MANNAN AND GLUCAN IN THE CELL-WALL OF CANDIDA-BOIDINII CULTIVATED ON METHANOL

The polysaccharide components (mannan and glucan) in the cell wall of Candida boidinii M 363 grown on methanol and glucose as control were i nvestigated using electron microscopy, cytochemical and biochemical me thods. An ultrastructural rearrangement of the polymers in the cell wa ll of yeasts cultivated on methanol in comparison to those cultivated on glucose was established. The morphological changes correlate to the quantitative changes in the polysaccharide constituents of the cell w all. The forming and the role of thiosemihydrocarbazide (TSHC) - negat ive zones in the Candida boidinii cell wall cultivated on methanol med ia are discussed.