UVB-induced decrease of p16/CDKN2A expression in skin cancer patients

Citation
G. Krahn et al., UVB-induced decrease of p16/CDKN2A expression in skin cancer patients, PIGM CELL R, 14(3), 2001, pp. 201-205
Citations number
32
Categorie Soggetti
Cell & Developmental Biology
Journal title
PIGMENT CELL RESEARCH
ISSN journal
08935785 → ACNP
Volume
14
Issue
3
Year of publication
2001
Pages
201 - 205
Database
ISI
SICI code
0893-5785(200106)14:3<201:UDOPEI>2.0.ZU;2-X
Abstract
The lack of p16 expression has been shown in cultured melanoma cells, howev er contradictory evidence for p16 expression in melanoma tissues exist. Ult raviolet (UV) C and UVB have been shown to affect p16 expression, which imp airs cell cycle regulation in vitro and in vivo, In this study, p16/CDKN2A gene expression was determined by reverse transcription polymerase chain re action in seven skin cancer patients, in one dysplastic nevus patient and i n seven healthy individuals, prior to UVB exposure and at various times aft er application of one minimal erythema dose (MED). Five of the seven skin c ancer patients showed a down-regulation of p16/ CDKN2A expression after UVB exposure, while controls remained unaltered. The UVB-induced decline of p1 6/ CDKN2A in skin cancer patients might offer new insights into photocarcin ogenesis. The putative sequence of events could start with a down-regulatio n of p16/CDKN2A expression, which would lead to impaired cell cycle regulat ion. Altered expression patterns of p16/CDKN2A following UVB exposure could be of value for identifying people with an increased risk of UV-induced sk in cancer.