Preparation and validation of an antibody against gibberellin glucosides

The preparation and the characteristics of an antibody specific for the 2-O -beta -D-glucosides of C-19-gibberellins are described. The antibody, anti- GA(26)-GEt-antibody, was prepared by immunizing rabbits with an immunogenic conjugate of 2-O-beta -D-glucosyl gibberellin A(26) (GA(26)-GEt) and bovin e serum albumin. The cross-reactivities of gibberellin glucosides with the anti-GA(26)-GEt-antibody were examined by enzyme-linked immunosorbent assay (ELISA). While 2-O-beta -D-glucosylated gibberellins such as GA(8)-GEt, GA (26)-GEt, and GA(29)-GEt showed high cross-reactivity to the antibody, othe rs did not. The logit-log standard plots showed the measuring range was fro m 30 fmol to 10 pmol for the GA(8)-GEt and GA(26)-GEt. The validity of the antibody was examined by the analysis of GA-GEts in immature seeds of Pharb itis nil.