Application of high-precision isotope ratio monitoring mass spectrometry to identify the biosynthetic origins of proteins

Authors
Apostol, I Brooks, PD Mathews, AJ
Citation
I. Apostol et al., Application of high-precision isotope ratio monitoring mass spectrometry to identify the biosynthetic origins of proteins, PROTEIN SCI, 10(7), 2001, pp. 1466-1469
Citations number
34
Categorie Soggetti
Biochemistry & Biophysics
Journal title
PROTEIN SCIENCE
ISSN journal
09618368 → ACNP
Volume
10
Issue
7
Year of publication
2001
Pages
1466 - 1469
Database
ISI
SICI code
0961-8368(200107)10:7<1466:AOHIRM>2.0.ZU;2-8
Abstract
Isotope ratio monitoring (IRM) mass spectrometry was used to measure the re lative abundance of stable isotopes in several samples of adult human hemog lobin expressed in E. coli, yeast, and human blood. The results showed sign ificant differences in the distribution of N-15 and C-13 isotopes among hem oglobin samples produced in these organisms. This indicates that IRM mass s pectrometry can be used in forensic protein chemistry to identify the origi n of protein expression.