Synthesis of a macroporous hydrophilic ternary copolymer and its application in boronate-affinity separation

A new macroporous ternary copolymer was synthesized using vinyl acetate (VA C), glycidyl methacrylate (GMA) and allyl methacrylate (AMA) through suspen sion polymerization with a mixture of n-heptane and butyl acetate as the po rogenic agent. The effects of the crosslinking degree, the level of GMA and the porogenic agent mixture and composition on the pore structure of the c opolymer and on the properties of the alcoholysed copolymer were investigat ed. The properties of a typical adsorbent were pore diameter 18.9 nm, pore volume 0.38 ml/g, and specific surface area 80.2 m(2)/g. A hydrophilic poly vinyl alcohol-based matrix was obtained on alcoholysis of the copolymer wit h the epoxy group unaffected. The alcoholysed copolymer was then attached t o a spacer, 6-aminocaproic acid (6-ACA), and finally coupled to 3-aminophen ylboronic acid (APBA) as a ligand. The addition reaction between the epoxy group of the matrix and the amino group of 6-ACA was also examined. The lig and density of the prepared affinity adsorbent was 0.865 mmol/g dry resin, which was applied to purify a polysaccharide peptide of Coriolus versicolor (PSP). The optimal conditions for the adsorption of PSP was 0.2 M ammonium acetate (pH 8.2) containing 0.2 M NaCl. (C) 2001 Published by Elsevier Sci ence B.V.