A novel gene from Myxococcus xanthus that facilitates membrane translocation of an extracellular endoglucanase in Escherichia coli?

Authors
Bensmail, L Monnier, C Quillet, L Guespin-Michel, JF Barray, S
Citation
L. Bensmail et al., A novel gene from Myxococcus xanthus that facilitates membrane translocation of an extracellular endoglucanase in Escherichia coli?, RES MICROB, 152(5), 2001, pp. 487-492
Citations number
25
Categorie Soggetti
Microbiology
Journal title
RESEARCH IN MICROBIOLOGY
ISSN journal
09232508 → ACNP
Volume
152
Issue
5
Year of publication
2001
Pages
487 - 492
Database
ISI
SICI code
0923-2508(200106)152:5<487:ANGFMX>2.0.ZU;2-9
Abstract
Expression in Escherichia coli of the Myxococcus xanthus gene celA, which e ncodes an extracellular endoglucanase, resulted in CelA being distributed b etween cytoplasm, periplasm and membrane. The presence of an adjacent open reading frame downstream from the full celA gene, or the absence of a putat ive lipoprotein signal sequence, confined CelA distribution to the periplas m and membrane, or to the cytoplasm and periplasm, respectively. (C) 2001 E ditions scientifiques et medicales Elsevier SAS.