Pseudomonas brenneri sp nov., a new species isolated from natural mineral waters

The vernacular name 'fluorescent Pseudomonas group 97-391' was coined for a group of 11 strains isolated from two French natural mineral waters. All t hese strains were Gram-negative, rod-shaped and motile by means of a single polar flagellum. They produced fluorescent pigment (pyoverdin) on King B m edium, catalase and cytochrome oxidase. They were not able to accumulate po ly-beta -hydroxybutyrate. They were capable of respiratory but not fermenta tive metabolism. DNA-DNA hybridization results and DNA base composition ana lysis revealed that strains of the 'fluorescent Pseudomonas group 97-391' w ere members of a new species, for which the name Pseudomonas brenneri sp. n ov. (type strain CIP 106646(T)) is proposed. The levels of DNA-DNA relatedn ess within this: group ranged from 70 to 100% with DeltaT(m) below 1 degree sC. The G+C content of the DNA of the type strain was 58 mol%. DNA relatedn ess with 72 strains representing well-known or partially characterized spec ies of the genus Pseudomonas (sensu stricto) was below 48%. The complete 16 S rRNA sequence of the type strain CIP 106646T was determined and compared with those of the type strains of Pseudomonas species. Finally, a phylogene tic tree was inferred from sequence analysis and demonstrated that the new species fell into the 'Pseudomonas fluorescens intrageneric cluster'. The c linical significance of P. brenneri is unknown. (C) 2001 Editions scientifi ques et medicales Elsevier SAS.