A novel multichannel surface plasmon resonance biosensor

We report a novel multichannel surface plasmon resonance (SPR) sensor based on the combination of parallel sensing channel architecture and the method of spectral discrimination of sensing channels. This sensor configuration provides multichannel performance and, through probing surface processes (e .g. binding of immobilized antibody and antigen in solution) with surface p lasmons with different field distributions, it allows distinguishing sensor response to surface and bulk effects, enabling more accurate quantificatio n of biomolecular interaction events. The potential of this mixed-architect ure sensor is illustrated in a model biosensing experiment in which the det ection of monoclonal anti-dinitrophenyl antibody (a-DNP) is compensated for non-specific adsorption and background refractive index interferences. (C) 2001 Elsevier Science B.V. All rights reserved.