A reciprocal translocation between 'Garfi' almond and 'Nemared' peach

A map with 51 markers ( 46 RFLPs and five isozymes) was constructed using a n interspecific F-2 population between 'Garfi' almond (Prunus amygdalus Bat sch.) and 'Nemared' peach [Prunus persica (L.) Batsch.]. This map was devel oped by selecting markers covering most of the distance of the eight linkag e groups from previously constructed Prunus maps. The markers studied in th is population mapped to seven linkage groups instead of the eight expected in Prunus. Markers belonging to groups 6 and 8 in previous maps formed a si ngle group in the 'Garfi' x 'Nemared' F-2 and several marker pairs placed i n different groups in other maps exhibited tight linkages. The study of pol len fertility and chromosome behavior during meiosis in the F-1 generation allowed us to confirm the hypothesis that a reciprocal translocation exists between 'Garfi' and 'Nemared'. Based on independent evidence of linkage be tween markers and pollen fertility data in the F-2 population, we concluded that the breakpoint of the reciprocal translocation was placed between mar kers AC50 and AG26A in group 6 and between markers AG112A and FG230A in gro up 8.