Pc. Binsfeld et al., Cytogenetic analysis of interspecific sunflower hybrids and molecular evaluation of their progeny, THEOR A GEN, 102(8), 2001, pp. 1280-1285
Meiotic cells of transgenic asymmetric somatic hybrid (ASH) plants obtained
by fusion of microprotoplasts of the donor species Helianthus giganteus or
Helianthus maximiliani and recipient protoplasts of Helianthus annuus were
investigated. Over 85% of the ASH meiocytes showed regular bivalent chromo
some pairing; however, several anomalies like anaphase bridges, laggard chr
omosomes, univalent and multivalent pairing were also observed. Pollen viab
ility of the ASH plants ranged from 79.2 to 95% with a strong negative corr
elation to chromosome number which varied between 34 and 42. Molecular inve
stigation of ASH progeny using RAPD markers revealed the presence of donor
genotype markers in 68% of the offspring. These results suggest that asymme
tric somatic hybridization offers an efficient alternative method to overco
me sexual barriers for gene flow and the genetic improvement of H. annuus b
y introgression of economical important traits from wild Helianthus species
.