Nuclei of plants as a sink for flavanols

Citation
W. Feucht et J. Polster, Nuclei of plants as a sink for flavanols, Z NATURFO C, 56(5-6), 2001, pp. 479-481
Citations number
12
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES
ISSN journal
09395075 → ACNP
Volume
56
Issue
5-6
Year of publication
2001
Pages
479 - 481
Database
ISI
SICI code
0939-5075(200105/06)56:5-6<479:NOPAAS>2.0.ZU;2-A
Abstract
Onion cepa (L.) and Tsuga canadensis (L.) Carr. were investigated histochem ically on the association of flavanols to nuclei. The young roots of Onion cepa are totally devoid of flavanol structures. Therefore, the excised root s tips were directly incubated into different solutions of flavanols. After 3 h of incubation a flavanol binding on the nuclei was recognizable, as se en by a yellowish-brown tanning reaction. Still to ensure the presence of f lavanols on the nuclei, subsequent staining with the p-dimethylaminocinnama ldehyde reagent (DMACA) resulted in an intense blue colouration. Tsuga cana densis has significant amounts of vacuolar flavanol deposits in all parts o f the tree as indicated by the DMACA reagent. It is obvious that also the n uclei were associated strongly with flavanols which can be demonstrated par ticularly elegant in the cells of the seed wings by histochemical methods. However, the mode of flavanol release from the original deposits is not yet clear.