Characterization of the extracellular enzyme systems of Trichoderma virideAH124

A mycoparasitic Trichoderma viride strain was investigated for the producti on of extracellular enzymes important in antagonism, by using natural and c hromogenic substrates. Some of these enzymes, such as beta -1,3-glucanases, and low levels of proteases were produced constitutively. Under inductive conditions, the measurable activities of beta -1,3-glucanase, protease and aspecific chitinase increased, while for the proteases and betaP-1,3-glucan ases, the levels depended on both the nitrogen and the carbon source. Gel f iltration chromatography revealed at least 4 beta -1,3-glucanases, 6 protea ses, 2 beta -glucosidases and 1 beta -1,4-N-acetyl-glucosaminidase izoenzym e under inductive conditions.