A. Svoboda et al., Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae, ACT BIOL HU, 52(2-3), 2001, pp. 325-333
The actin cables and microtubules were disrupted during protoplasting in Sa
ccharomyces cerevisiae cells. In the process of protoplast regeneration, th
e cytoplasmic microtubules were the first to be restored; the actin patches
remained regularly distributed under the surface of the growing protoplast
. After the cell wall had been regenerated in a gelatine medium, the actin
patches aggregated into clusters, which marked the site of bud development.
An incomplete cell wall was the apparent cause for uncoupling between kary
okinesis and cytokinesis. The presence of several nuclei in the cell gave r
ise to several buds emerging simultaneously and was probably related to the
ir random positions. in haploids, however, the axial type of budding was al
so obvious in the reverting protoplasts. These observations suggest that ac
tin is a structure involved in the restoration of polar growth during proto
plast regeneration, and that the cell wail plays an important role in this
process: in its absence, actin fails to polarise.