K. Johnson et al., Interleukin-1 induces pro-mineralizing activity of cartilage tissue transglutaminase and factor XIIIa, AM J PATH, 159(1), 2001, pp. 149-163
Citations number
59
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Two transglutaminases (TGases), Factor XIIIa and tissue TGase (tTGase), are
expressed in temporal-spatial association with matrix calcification In gro
wth plates. Meniscal and articular cartilage matrix calcification are preva
lent in osteoarthritis (OA) and aging. Here, we demonstrated up-regulation
of tTGase and Factor XIIIa in superficial and deep zones of knee OA articul
ar cartilage and the central (chondrocytic) zone of OA menisci. Transformin
g growth factor-beta and interleukin (IL)-1 beta Induced Factor XIIIa and t
TGase expression in cartilage and meniscal organ cultures. Thus, we studied
TGase activity. Donor age-dependent, OA severity-related, and IL-1-induced
increases in TGase activity were demonstrated in both knee menisci and cul
tured meniscal cells. Meniscal cell TGase activity was stimulated by nitric
oxide donors and tumor necrosis factor-alpha, but transforming growth fact
or-beta did not stimulate TGase activity. The iNOS inhibitor N-monomethylar
ginine (NMMA) and an Inhibitor of tumor necrosis factor receptor-associated
factor (TRAF)2 and TRAF6 signaling (the zinc finger protein A20) suppresse
d IL-1 Induction of TGase activity. Increased Factor XIIIa and tTGase activ
ities, achieved via direct transfection of chondrocytic TC28 and meniscal c
ells, both induced matrix apatite deposition. Thus, Factor XIIIa and tTGase
activities were increased in aging, degenerative cartilages and induced by
IL-1. Because TGase activity promoted apatite deposition, our findings pot
entially implicate inflammation in the pathogenesis of cartilage matrix cal
cification.