Denitrification losses show an irregular pattern through the year, oft
en being caused by climatic conditions and management practices. The o
bjectives of the present work were to quantify denitrification losses
and to determine the influence of tillage system on the factors that c
ontrol denitrification in fertilized soils. The modal profile of the s
oil was an Vertic Argiudoll, clay loam texture, located in Buenos Aire
s province, Argentina. The treatments were: (a) fertilized, (b) incorp
orated fertilization and (c) without fertilization for both no tillage
and conventional tillage systems. Chambers were placed in the field t
o measure denitrification. In this clayish soil the estimated mean val
ues of accumulated denitrification during the crop cycle (90 days) wer
e 0.190 kg N ha(-1) for conventional tillage and 0.350 kg N ha(-1) for
no tillage. In treatments with no tillage, losses by denitrification
were approximately twice those of conventional tillage. These differen
ces were also evidenced by the number of microorganisms, which were si
gnificantly higher (P<5%) for no tillage on all dates, except for at f
lowering. The increase at flowering coincided with the period of highe
st rainfall and consequently the highest water contents in the soil. T
he highest denitrification losses, except for sowing, were measured wh
en soil moisture content was more than 30% (v/v). Denitrification incr
eased in conjunction with an increase in the availability of carbon th
at is consumed by the heterotrophic microorganisms (including the deni
trifiers).