Decrease in Cryptosporidium parvum oocyst infectivity in vitro by using the membrane filter dissolution method for recovering oocysts from water samples

Authors
Carreno, RA Pokorny, NJ Weir, SC Lee, H Trevors, JT
Citation
Ra. Carreno et al., Decrease in Cryptosporidium parvum oocyst infectivity in vitro by using the membrane filter dissolution method for recovering oocysts from water samples, APPL ENVIR, 67(7), 2001, pp. 3309-3313
Citations number
26
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
67
Issue
7
Year of publication
2001
Pages
3309 - 3313
Database
ISI
SICI code
0099-2240(200107)67:7<3309:DICPOI>2.0.ZU;2-X
Abstract
Exposure of Cryptosporidium parvum oocysts to solutions used for cellulose acetate membrane (CAR I) dissolution filtration reduced their infectivity i n HCT-8 cells. Ethanol (95% [vol/vol] and 70% [vol/vol]) alone and short ex posure times to acetone decreased infectivity. These findings contrast with similar experiments using excystation assays and infectivity in mice.