Liposome supported leukocyte cultures and standardisation for zytogenetic preparations

There are considerable difficulties in making constant and qualitatively go od Chromosomal preparations from breeding animals. Varying animal material (without special management information), ingredients of the media, mitogen es, or different qualities of fetal calf serum are all influencing factors. We made experiments with liposomes, which have shown in the literature to improve the quality of chromosomal preparations. There are only a few exami nations reported about optimizing the culture conditions for short term cul tures, including the following chromosomal preparations as well. These experiments have shown that fetal calf serum cannot be replaced by li posomes and the mitogene is obligatory for metaphases-.