K. Huch, Knee and ankle: human joints with different susceptibility to osteoarthritis reveal different cartilage cellularity and matrix synthesis in vitro, ARCH ORTHOP, 121(6), 2001, pp. 301-306
Clinical experience shows that symptoms and pathological changes of primary
osteoarthritis (OA) are more frequent and severer in the knee than in the
ankle joint. The different anatomy of both weight-bearing joints implies th
at biomechanical differences may contribute to their varying susceptibility
to OA. This study aims at elucidating other non-biomechanical factors to e
xplain these fundamental differences in secondary OA prevalence. Human cart
ilage of matched ankle and knee joints from organ donors was dissected in f
ull-thickness slices or in layers. The DNA content for estimation of cell n
umber was analyzed fluorometrically. Chondrocytes were cultured in organ cu
lture or after isolation in alginate. Proteoglycan synthesis was determined
by S-35 incorporation, and collagen synthesis by H-3-proline incorporation
. This study demonstrates that in both joints, the cell density sharply dec
lines between newborn and young infant ages. In addition, cartilage from th
e ankle joint is significantly more cellular than cartilage from the knee j
oint. In general, ankle chondrocytes synthesize more proteoglycans (PGs) an
d collagens than knee chondrocytes, and deep zone chondrocytes more than su
perficial zone chondrocytes. The biochemical properties of chondrocytes of
the ankle and knee joints differ significantly and might play an important
role in the pathogenesis of OA.