Detection of infectious viral particles in plant protoplasts inoculated with transcripts of full-length shallot virus X cDNA - Brief report

Authors
Vishnichenko, VK Zavriev, SK
Citation
Vk. Vishnichenko et Sk. Zavriev, Detection of infectious viral particles in plant protoplasts inoculated with transcripts of full-length shallot virus X cDNA - Brief report, ARCH VIROL, 146(6), 2001, pp. 1213-1217
Citations number
9
Categorie Soggetti
Microbiology
Journal title
ARCHIVES OF VIROLOGY
ISSN journal
03048608 → ACNP
Volume
146
Issue
6
Year of publication
2001
Pages
1213 - 1217
Database
ISI
SICI code
0304-8608(2001)146:6<1213:DOIVPI>2.0.ZU;2-1
Abstract
Flexible filamentous shallot virus X (ShVX) particles were detected in extr acts of Bern vulgaris protoplasts inoculated with transcripts from a full-l ength ShVX cDNA, Extracts from ShVX-infected protoplast were infectious for ShVX-healthy shallot seedlings. Western blot analysis of inoculated plants revealed the accumulation of the ShVX coat protein, while electron microsc opy confirmed the presence of ShVX virions. The results suggest that the in vitro RNA transcripts from full-length ShVX cDNA give rise to infectious v iral particles.