Increase of ceramides and its inhibition by catalase during chemically induced apoptosis of HL-60 cells determined by electrospray ionization tandem mass spectrometry
Y. Yamada et al., Increase of ceramides and its inhibition by catalase during chemically induced apoptosis of HL-60 cells determined by electrospray ionization tandem mass spectrometry, BBA-MOL C B, 1532(1-2), 2001, pp. 115-120
Citations number
20
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
A change in all ceramide species during chemically induced apoptosis of HL-
60 cells was determined using electrospray tandem mass spectrometry. Cerami
des of C16:0, C24:1 and C26:1 increased significantly 4 h after the additio
n of actinomycin D, when the activation of caspase-3 was maximal. Addition
of catalase, which inhibited apoptosis, the activation of caspase-3-like pr
otease, and the release of cytochrome c from mitochondria to cytosol caused
by actinomycin D or daunorubicin, significantly inhibited the increase of
these ceramides at all time points. Ceramides of C16:0, C24:1, C18:0, C22:1
and C26:1 increased significantly 4 h after the addition of daunorubicin t
o HL-60 cells. Catalase also significantly inhibited the increase of these
ceramides induced by daunorubicin. Based on time courses of events and inhi
bition studies, it is concluded that the increase of ceramides is downstrea
m from both generation of hydrogen peroxide and cytochrome c release from m
itochondria and takes place almost simultaneously with the activation of ca
spase-3. (C) 2001 Elsevier Science B.V. All rights reserved.