Copper staining method for extracting biologically active proteins from native gels

Authors
Wen, J Zhang, M Horan, TP Philo, JS Li, TS Wypych, J Mendiaz, EA Langley, KE Aoki, KH Kuwamoto, M Kita, Y Arakawa, T
Citation
J. Wen et al., Copper staining method for extracting biologically active proteins from native gels, BIOS BIOT B, 65(6), 2001, pp. 1315-1320
Citations number
17
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
65
Issue
6
Year of publication
2001
Pages
1315 - 1320
Database
ISI
SICI code
0916-8451(200106)65:6<1315:CSMFEB>2.0.ZU;2-0
Abstract
An attempt was made to make protein bands visible on native gel using coppe r staining, since such a mild staining procedure would make the entire nati ve gel electrophoresis process non-denaturing, Copper staining not only was able to detect various proteins on native gel with reasonable sensitivity, but also made extraction and recovery of active proteins possible from the gel using a gentle procedure.