Conjugation of Arg-Gly-Asp (RGD) sequence in copolymer bearing sugar moiety for insulinoma cell line (MIN6) culture

Copolymers composed of an Arg-Gly-Asp (RGD) sequence for the adhesion molec ule and sugar moieties were synthesized for an insulinoma cell (MIN6) cultu re, MIN6 cells attached on the poly( N-p-vinylbenzyl-D-maltonamide-co-6-(p- vinylbenzamido)-hexanoic-acid-g-GRGDS) (p(VMA-co-VBGRGDS))-coated dishes we re in a more aggregated form than other polymer-coated surfaces, P(VMA-co-V BCRCDS) also shows faster proliferation of MIN6 cells (about 18% higher) th an with p(VLA-co-VBGRGDS), By interaction between cell and matrix, about 80 % greater insulin secretion from MIN6 cells was produced with the p(VMA-co- VBGRGDS), and about 50% greater insulin secretion was produced with the pol y( N-p-vinylbenzyl-Dlactonamide-co-6-(p-vinylbenzamido)-hexanoic acid-g-GRG DS) (p(VLA-co-VBGRGDS) as compared with unstimulated cells. Moreover, attac hment of MIN6 cells treated with RGD monomer was suppressed approximately 5 0% for the p(VMA-co-VBGRGDS) surface. This result supported the idea that c onjugation of adhesion molecules of RGD peptide in p(VMA-coVBGRGDS) copolym er specifically interact with integrin families on MIN6 cell membrane.