Postischemic myocardial recovery and oxidative stress status of vitamin C deficient rat hearts

Objective: To investigate the role of vitamin C tissue content as a protect ive agent during myocardial ischemia-reperfusion injury. we have evaluated the postischemic Functional recovery and free radical release of osteogenic disorder Shionogi (ODS) inherently scorbutic rat hearts and compared them to healthy Wistar rat hearts. Methods: Isolated perfused hearts of ODS or W istar rats underwent 30 min of a global total normothermic ischemia followe d by 30 min of reperfusion. The lipid-soluble spin trap alpha -phenyl N-ter t-butylnitrone (3 mM) was perfused upstream of the coronary bed. Functional parameters were recorded and samples of coronary effluents were analysed u sing electron spin resonance spectroscopy to characterise and quantify the amount of radical species released. Results: From the onset of reperfusion, a large and long-lasting release of alkyl/alkoxyl radicals was detected, w ith a peak value of 29.0+/-3.2 nM obtained after 13 min, which was associat ed with a persistent contractile dysfunction. However, ODS rat hearts showe d a higher myocardial recovery with lower left ventricular end diastolic pr essure (44.34+/-1.74 vs. 55.03+/-1.57 mmHg for Wistar), higher recovery of rate pressure product (12.3+/-1.4 vs. 1.9+/-1.7x10(3) mmHg beats/min for Wi star) and shorter duration of contractile abnormalities during reperfusion (3.7+/-1.0 vs. 20.8+/-5.3 min for Wistar). Moreover, free radical release w as identical in ODS rat hearts as compared to control Wistar rats. Ascorbic acid tissue content was significantly altered in ODS rats (31.9+/-3.3 vs. 591.0+/-54.9 mmol/g of tissue for Wistar) but superoxide dismutases, glutat hion peroxidases and inducible heat shock protein 70 genes were up-regulate d. Conclusions: This study shows that ascorbic-acid-deficient ODS rat heart s are more resistant to an ischemic insult than control Wistar rats, probab ly through the development of alternative protective defences. like the ind uction of heat shock proteins. These paradoxical results raise the question of the relative importance of each endogenous antioxidant in the cardiac r esistance to ischemia-reperfusion injury. (C) 2001 Elsevier Science B.V. Al l rights reserved.