Studies on the catalytic spectrophotometry using SDS-Schiff Cu(II) complexas mimetic enzyme

A new Schiff base, 2-hydroxy-1-napthaldehyde-2-amino-thiazole (HNATS) and i ts Cut II) complex was synthesized. The complex [Cu(I)-(HNATS)(2)] could ex hibit an efficient peroxitase-like catalytic activity, and catalyze the cou pling reaction of 2,4-dichlorophenol and 4-amino antipyrine with hydrogen p eroxide. The effect of SDS on the catalytic activity of mimetic enzyme was studied. Based on the reaction, the determination of O-2(.-) by this new sp ectrophotometric method was developed. The proposed method was used to dete rmine SOD activity of garlic, scallion and onion with satisfactory results.