Spectroscopic characteristics of RTP palladium-porphyrin probe interactingwith DNA in aqueous solution

The characteristics of absorption, fluorescence, room temperature phosphore scence(RTP) spectra of meso-tetrakis( 4-N-trimethylaminobenzyl)porphin (TAP P) and Palladium-porphyrin (Pd-TAPP) probe in the presence and absence of c t-DNA has been studied. Pd-TAPP shows a very weak RTP emission in aqueous s olution and strong RTP emission in the presence of DNA. Maximum RTP was obs erved at pH=7, with maximum excitation and emission wavelengths at 414 nm a nd 684 nm, respectively. RTP decay follows a first order exponential equati on, RTP lifetime (tau) of probe is (0.72 +/-0.03) ms. The detection limit o f ct-DNA may reach 8.8 X 10(-7) mol/L. The relative standard deviation(RSD) is less than +/- 6.6% in the linear range.