Detection and removal of an artefact fatty acid from the binding site of recombinant Bombyx mori pheromone-binding protein

Authors
Oldham, NJ Krieger, J Breer, H Svatos, A
Citation
Nj. Oldham et al., Detection and removal of an artefact fatty acid from the binding site of recombinant Bombyx mori pheromone-binding protein, CHEM SENSE, 26(5), 2001, pp. 529-531
Citations number
12
Categorie Soggetti
Multidisciplinary,"Neurosciences & Behavoir
Journal title
CHEMICAL SENSES
ISSN journal
0379864X → ACNP
Volume
26
Issue
5
Year of publication
2001
Pages
529 - 531
Database
ISI
SICI code
0379-864X(200106)26:5<529:DAROAA>2.0.ZU;2-1
Abstract
Recombinant Bombyx mori pheromone-binding protein (PBP), purified from an E scherichia coil expression system, has been found to contain (11Z)-octadece noic acid (cis-vaccenic acid) as an artefact ligand. An efficient delipidat ion procedure is described to overcome what would appear to be a general pr oblem with recombinant lepidopteran PBPs.