Development of a simple whole blood panel test for detection of human heart-type fatty acid-binding protein

Objective: For the diagnosis of acute myocardial infarction (AMI), we have developed a rapid and simple whole blood panel test for the detection of hu man heart-type fatty acid-binding protein (H-FABP) using one-step immunochr omatography. Methods and Results: We have developed a whole blood panel test for rapid d etection of human H-FABP using a one-step immunochromatography technique. T he result of this panel test was not affected by the other contents of the blood such as bilirubin, hemoglobin and others. Furthermore, no cross-react ivity of the antibodies was found with other cardiac markers or other tissu e-type FABPs. The result of this panel test was similar to the diagnostic c ut-off value, 6.2 ng of H-FABP per Int of serum which was evaluated by the enzyme-linked immunosorbent assay (ELISA), Conclusion: We have developed a simple one-step immunochromatography techni que to detect H-FABP in whole blood sample. Further studies are required to identify the value of this point-of-care testing (POCT) as a diagnostic ma rker for AMI. (C) 2001 The Canadian Society of Clinical Chemists. All right s reserved.