T. Yamada et al., DIETARY DIOSGENIN ATTENUATES SUBACUTE INTESTINAL INFLAMMATION ASSOCIATED WITH INDOMETHACIN IN RATS, American journal of physiology: Gastrointestinal and liver physiology, 36(2), 1997, pp. 355-364
We investigated the effects of dietary diosgenin (Die), a plant-derive
d sapogenin, on indomethacin (Indo)-induced intestinal inflammation an
d alterations in bile secretion in rats. In anesthetized rats, bile se
cretion, intestinal inflammation, and blood chemistry were assessed 3
days after two subcutaneous injections of Indo given 24 h apart. Dio (
>80 mg.kg(-1).day(-1)) pretreatment significantly inhibited weight and
food intake decreases and intestinal inflammation. This protective ef
fect was confirmed by examination of gross and histological findings a
nd intestinal myeloperoxidase activity. Dio significantly increased bi
liary cholesterol (Chol) output and prevented the decreases in bile fl
ow, bile acid output, and biliary alpha-muricholic acid and the increa
ses in biliary hyodeoxycholic acid, deoxycholic acid, and hydrophobici
ty index of bile. Significantly more biliary Chol and phospholipids we
re present in macromolecules separate from bile acids and Indo in Die-
treated rats. Dio significantly increased the elimination constant of
Indo and reduced plasma Indo levels at 3 and 12 h but did not influenc
e biliary secretion of Indo for 3.5 h after injection. Although Dio do
se-dependently attenuated subacute intestinal inflammation and normali
zed bile secretion in this model, it may also compromise the anti-infl
ammatory action of Indo.