Lentinula edodes produces a multicomponent protein complex containing manganese (II)-dependent peroxidase, laccase and beta-glucosidase

A multicomponent protein complex containing manganese (II)-dependent peroxi dase, laccase and beta -glucosidase was isolated from culture extracts of t he white rot basidiomycete Lentinula edodes. This protein complex showed a single protein band on native polyacrylamide gel electrophoresis (PAGE). On sodium dodecyl sulfate (SDS) PAGE, however, it displayed three major bands and several additional minor bands ranging in size from 60 kDa to 180 kDa, suggesting it being a complex of six to right different proteins. The mole cular mass of this complex was estimated to be approximately 660 kDa from t he elution position of gel filtration. This enzyme complex was effective in transforming environmentally persistent xenobiotics. pentachlorophenol and 2-5-dichlorophenol. (C) 2001 Federation of European Microbiological Societ ies. Published by Elsevier Science B.V. All rights reserved.