Spectrophotometric analysis of porphyrin for quantative determination of poultry haemoglobin

A method improved by NEUMANN (1999) for the quantitative analysis of poultr y haemoglobin following conversion to porphyrin is described. The specimen materials used were shaken blood and heparinised blood of broiler as well a s crystalline pigeon haemoglobin. The studies included the examination of t hese specimen materials for their suitability for this method, the conversi on of defined haemoglobin solutions (native and crystalline haemoglobins) t o porphyrins, and their spectrophotometric analysis. The loss of haemoglobi n in the process of ultrafiltration through a Millipore filter was determin ed and used to calculate a correction value. The regression equation derive d on the grounds of the results enabled an unknown haemoglobin concentratio n to be calculated from a measured extinction.