Barley chromosome addition lines of wheat for screening of AFLP markers onbarley chromosomes

We conducted AFLP (Amplified Fragment Length Polymorphism) analysis with th e six wheat-barley chromosome addition lines of common wheat cultivar Chine se Spring. We analyzed the AFLP fingerprints generated by 36 combinations o f selective-amplification primers to find 103 markers specific to the barle y chromosomes (2.9 markers per combination on average). The numbers of AFLP markers mapped to the barley chromosomes varied tone to 161 depending of t he primer combinations. Each barley chromosome had 10 to 27 AFLP markers (1 7.2 markers on average). We identified the chromosome arms in which these m arkers are located using the barley telocentric addition lines tone to 20 m arkers per chromosome arm). The AFLP markers were not distributed evenly am ong chromosomes and chromosome arms. We could not determine the chromosome- arm locations for some of the barley-specific markers, either because such markers were found in both the short- and long-arm telocentric lines, or in neither line.