The comparison of electron microscopy and scintigraphy in determining the protective effect of dimethylsulphoxide (DMSO) on ischemia/reperfusion injury through pringle maneuver

Background/Aims: We investigated the role of the electron microscopy and he patobiliary scintigraphy in determining the effect of DMSO (dimethysulphoxi de) and ischemia/reperfusion injury in the liver after the Pringle maneuver . Methodology: Twenty-four rabbits were divided into the following groups; A: Control group, B: Pringle, C: 10mg/kg DMSO, D: 1g/kg DMSO + Pringle. Group A was considered as a control group and only laparotomy was applied. Group B was exposed to Pringle maneuver only. Group C:was given 10mg/kg of DMSO via the vena cava inferior. Group D was given 1g/kg of DMSO- A clamp was fa stened for the groups of B, C and D in the 30th minute of the Pringle maneu ver and a biopsy was applied five minutes later. Fifteen minutes later a dy namic hepatobiliary scintigraphy was applied. From dynamic images, liver pe ak time and activity half time of the liver were obtained. Results: It was found that liver peak time and liver activity half time val ues of the group B, C and D were significantly longer than group A. Liver p eak time and liver activity half time values of group B was not different f rom group C. However, some values of group D were found to be significantly shorter than groups B and C. In the electron microscopy examination, only in group B were some specific degenerative changes observed in the sinusoid s. We observed less irreversible changes in group C than in group B. Oh the other hand, the least irreversible changes were in group D. Conclusions: As a conclusion, while electron microscopy is regarded as the "gold standard", hepatobiliary scintigraphy may be thought of as an easily applicable: method in determining the ischemic reperfusion injury in the cl inical comparison of the protective agents.