Effects of 22K or 20K human growth hormone on lipolysis, leptin productionin adipocytes in the presence and absence of human growth hormone binding protein

Method: We studied the effects of human growth hormone (hGH) on leptin prod uction and lipolysis stimulation in the presence or absence of human growth hormone binding protein (hGHBP) using 3T3-L1-hGHR adipocytes which efficie ntly express human growth hormone receptor. Results and Conclusion: It was clarified that (1) hGH decreases leptin secretion after hGH-induced lipolys is stimulation, and (2) the reduction of leptin production and lipolysis st imulation by 22K hGH was attenuated with hGHBP, whereas that by 20K hGH, wh ich is a naturally occurring isoform of 22K hGH, was not affected with hGHB P. Copyright (C) 2001 S. Karger AG. Basel.