Susceptibility testing of Trichophyton rubrum and Microsporum canis to three azoles by E-test

The purpose of our study was to assay the E-test for the determination of m inimum inhibitory concentration (MIC) of fluconazole, ketoconazole and itra conazole for antifungal susceptibility testing of 83 samples of Trichophyto n rubrum and Microsporum canis. For each sample, a mycelium suspension was prepared and inoculated in Petri dishes containing Sabouraud dextrose agar. Incubation was carried out at 25 degreesC for five to seven days for T. ru brum and three to five days for M. canis. The MIC values of fluconazole wer e > 256 mu mg/ml for most samples (96.1 %), while the MIC values of ketocon azole were > 32 mu mg/ml for 44.6 % of them and itraconazole presented MIC value > 32 mu mg/ml for only one isolate. Regarding its performance, the E- test was not labor demanding, was easy to interpret, and with the potential of being used as an alternative assay for azole antifungal susceptibility testing of T. rubrum and M. canis.