Electrophoretic behavior of peptides in capillary electrophoresis - Influence of ionic strength and pH in aqueous-organic media

Through correct pH, pK(a) and activity coefficients values, a model describ ing the effect of pH on electrophoretic mobility of substances has been app lied to a series of peptides in water and in acetonitrile-water mixtures. T he derived equations permit prediction of the optimum pH for the electropho retic separation from only a few experimental values and they also permit d etermination of pK(a) values of analytes in the aqueous-organic media emplo yed. Furthermore, the electrophoretic resolution between pairs of substance s can be predicted, in order to evaluate electrophoretic separations of the studied peptides. (C) 2001 Elsevier Science B.V. All rights reserved.