W. Buzina et al., Development of molecular methods for identification of Schizophyllum commune from clinical samples, J CLIN MICR, 39(7), 2001, pp. 2391-2396
In the last 50 years, to our knowledge, only 16 cases of diseases caused by
Schizophyllum commune in humans have been reported. Within only 6 months,
we found four isolates of this basidiomycetous fungus, obtained from patien
ts suffering from chronic sinusitis. The cultures of the isolated fungi sho
wed neither clamp connections nor fruiting bodies (basidiocarps), which are
distinctive features for S. commune, but fast-growing cottony white myceli
um only, This was harvested, and DNA was extracted. The internal transcribe
d spacer region of the ribosomal DNA (rDNA) was amplified with fungus-speci
fic primers, and the PCR products were sequenced. Two strains of S. commune
, collected from branches of a European hornbeam (Carpinus betulus) and a t
ree of heaven (Ailanthus altissima), respectively; four specimens from the
herbarium of the Institute of Botany, Karl-Franzens-University Graz; and tw
o strains from internationally known culture collections (CBS 340.81 [ATCC
44201] and CBS 405.96) were investigated in the same way. The sequence data
of all strains were compared and showed homology of over 99% in this 660-b
p-long fragment of rDNA. With these results, a map of restriction enzyme cu
tting sites and a primer set specific for S. commune were created for relia
ble identification of this human pathogenic fungus.