Use of real-time quantitative PCR to detect Chlamydophila felis infection

Authors
Helps, C Reeves, N Tasker, S Harbour, D
Citation
C. Helps et al., Use of real-time quantitative PCR to detect Chlamydophila felis infection, J CLIN MICR, 39(7), 2001, pp. 2675-2676
Citations number
9
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
39
Issue
7
Year of publication
2001
Pages
2675 - 2676
Database
ISI
SICI code
0095-1137(200107)39:7<2675:UORQPT>2.0.ZU;2-P
Abstract
A real-time PCR assay was developed to detect and quantify Chlamdophila fel is infection of cats. The assay uses a molecular beacon to specifically ide ntify the major outer membrane protein gene, is highly reproducible, and is able to detect fewer than 10 genomic copies.