Electrophoretic mobility anomalies associated with PCR amplification of the intergenic spacer region between 16S and 23S ribosomal RNA genes of Fusobacterium necrophorum

Authors
Narayanan, SK Nagaraja, TG Chengappa, MM Stewart, GC
Citation
Sk. Narayanan et al., Electrophoretic mobility anomalies associated with PCR amplification of the intergenic spacer region between 16S and 23S ribosomal RNA genes of Fusobacterium necrophorum, J MICROB M, 46(2), 2001, pp. 165-169
Citations number
20
Categorie Soggetti
Biology,Microbiology
Journal title
JOURNAL OF MICROBIOLOGICAL METHODS
ISSN journal
01677012 → ACNP
Volume
46
Issue
2
Year of publication
2001
Pages
165 - 169
Database
ISI
SICI code
0167-7012(200108)46:2<165:EMAAWP>2.0.ZU;2-5
Abstract
PCR amplification of the intergenic spacer region (ISR) between 16S and 23S rRNA genes among subspecies of the anaerobic bacterium Fusobacterium necro phorum gave identical patterns, with two forms of ISR identified. However, extra bands resulting from anomalous electrophoretic mobility of amplified DNA fragments with certain primer combinations were encountered. Therefore, PCR assays relying solely on banding patterns may be unreliable, and suppo rting sequence analysis is essential for correct culture identification. (C ) 2001 Elsevier Science B.V. All rights reserved.