Gas production by feces of infants

Background: Intestinal gas is thought to be the cause abdominal discomfort in infants. Little is known about the type and amount of gas produced by th e infant's colonic microflora and whether diet influences gas formation. Methods: Fresh stool specimens were collected from 10 breast-fed infants, 5 infants fed a soy-based formula, and 3 infants fed a milk-based formula at approximately 1, 2, and 3 months of age. Feces were incubated anaerobicall y for 4 hours at 37 degreesC followed by quantitation of hydrogen (H-2), me thane (CH4). carbon dioxide (CO2), hydrogen sulfide (H2S), methanethiol (CH 3SH), and dimethyl sulfide (CH3SCH3) in the headspace. Results: H-2 was produced in greater amounts by breast-fed infants than by infants in either formula group, presumably the consequence of incomplete a bsorption of breast milk oligosaccharides. CH4 was produced in greater amou nts by infants fed soy formula than by infants on other diets. CO2 was prod uced in similar amounts by infants in all feeding groups. Production of CH3 SH was conspicuously low by feces of breast-fed infants and production of H 2S was high by soy-formula-fed infants. CH3SCH3 was not detected. Only mode st changes with age were observed and there was no relation between gas pro duction and stool consistency, although stools were more likely to be malod orous when concentrations of H2S and/or CH3SH were high. Conclusions: Gas release by infant feces is strongly influenced by an infan t's diet. Of particular interest are differences in production of the highl y toxic sulfur gases, H2S and CH3SH, because of the role that these gases m ay play in certain intestinal disorders of infants.