A comparative study of raw and prepared semen samples from two consecutivedays

OBJECTIVE: To study and compare raw and prepared semen samples from two con secutive days by conventional sperm parameters, various motility characteri stics of spermatozoa determined by computer-aided sperm analysis and calciu m ionophore-induced acrosome reaction. STUDY DESIGN: Semen samples of male partners in couples undergoing 81 cycle s of double intrauterine insemination were studied. The first sample was pr oduced after abstinence of 2-7 days and the second, 24 hours after the firs t. Both samples were processed by isolate sperm separation medium. RESULTS: Semen volume, sperm concentration and total motile spermatozoa wer e significantly reduced in day 2 raw and prepared samples, whereas normal m orphology, motility characteristics and percentage of acrosome-reacted sper matozoa increased significantly in day 2 inseminated samples as compared to day 1. Oligospermic, asthenospermic and teratozoospermic samples showed a significant improvement in concentration, various motility characteristics and normal morphology of spermatozoa in day 2 samples as compared to day 1. CONCLUSION Men with normal samples showed improvements in normal morphology and acrosome-reacted spermatozoa, whereas those with subnormal semen sampl es from day 1 showed a significant improvement in concentration, various sp ermatozoal velocities and normal morphology on day 2.