Possibilities of flow cytometric analysis for immunophenotypic characterization of canine lymphoma

The purpose of the study here is to show the possibilities of flow cytometr ic analysis for immunophenotypic characterization of canine malignant lymph oma. Aspiration biopsies from lymph nodes of 30 dogs with multicentric lymp homa were stained by four dog-specific antibodies and three human-specific antibodies cross-reacting with canine lymphocytes, and analysed by flow cyt ometry. Twenty of the 30 lymphoma examined were characterized as B-cell-lym phoma and 10 as T-cell-lymphoma. Flow cytometry is a suitable method for th e immunophenotypic analysis of canine lymphoma. Contrary to immunohistochem istry, no surgically produced lymph node biopsy is required and the results are available within a few hours.