High-throughput yeast two-hybrid assays for large-scale protein interaction manning

Protein-protein interactions play fundamental roles in many biological proc esses. Hence, protein interaction mapping is becoming a well-established fu nctional genomics approach to generate functional annotations for predicted proteins that so far have remained uncharacterized. The yeast two-hybrid s ystem is currently one of the most standardized protein interaction mapping techniques. Here, we describe the protocols for a semiautomated, high-thro ughput, Gal4-based yeast two-hybrid system. (C) 2001 Academic Press.