Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms

Citation
S. Mion et al., Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms, MOL CELL NE, 17(6), 2001, pp. 957-972
Citations number
47
Categorie Soggetti
Neurosciences & Behavoir
Journal title
MOLECULAR AND CELLULAR NEUROSCIENCE
ISSN journal
10447431 → ACNP
Volume
17
Issue
6
Year of publication
2001
Pages
957 - 972
Database
ISI
SICI code
1044-7431(200106)17:6<957:BRONEB>2.0.ZU;2-7
Abstract
Alternative splicing in the mGluR5 gene generates two different receptor is oforms, of which expression is developmentally regulated. However, little i s known about the functional significance of mGluR5 splice variants. We hav e examined the functional coupling, subcellular targeting, and effect on ne uronal differentiation of epitope-tagged mGluR5 isoforms by expression in n euroblastoma NG108-15 cells. We found that both mGluR5 splice variants give rise to comparable [Ca2+](i) transients and have similar pharmacological p rofile. Tagged receptors were shown by immunofluorescence to be inserted in the plasma membrane. In undifferentiated cells the subcellular localizatio n of the two mGluR5 isoforms was partially segregated, whereas in different iated cells the labeling largely redistributed to the newly formed neurites . Interestingly, we demonstrate that mGluR5 splice variants dramatically in fluence the formation and maturation of neurites; mGluR5a hinders the acqui sition of mature neuronal traits and mGluR5b fosters the elaboration and ex tension of neurites. These effects are partly inhibited by MPEP.