Allele deletion mapping of the short arm of human chromosome 3 in renal carcinoma

Allelic deletions along the short arm of human chromosome 3 were mapped in 57 pairs of DNA samples from tumor and normal tissue of renal carcinoma pat ients in order to locate potential tumor suppressor genes. Twenty highly po lymorphic microsatellite markers were used for deletion mapping. Allelic de letions wel e found in most of the samples (91%). Extended terminal deletio ns (56%) prevailed over shorter internal and multiple: deletions and domina ted (65%) in the most aggressive histopathological kidney cancer subtype, d ear-cell carcinoma. Frequency analysis of loss of heterozygosity allowed de tection of the human chromosome 3 regions most essential for renal carcinom as: tile region adjacent to the gene VHL (3p26-p25), the region of homozygo us deletions AP20 (3p22-p21.33), acid a new region between markers D3S2420 and D3S2409 (3p21.31,2.2 Mbp).