Identification of the protease-binding domain in the N-terminal region of influenza virus A matrix protein M1

Authors
Timofeeva, TA Klenk, HD Zhirnov, OP
Citation
Ta. Timofeeva et al., Identification of the protease-binding domain in the N-terminal region of influenza virus A matrix protein M1, MOL BIOL, 35(3), 2001, pp. 411-416
Citations number
21
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR BIOLOGY
ISSN journal
00268933 → ACNP
Volume
35
Issue
3
Year of publication
2001
Pages
411 - 416
Database
ISI
SICI code
0026-8933(200105/06)35:3<411:IOTPDI>2.0.ZU;2-Q
Abstract
A region responsible for protease binding by influenza virus A matrix prote in M1 was identified. Trypsin binding was observed with the N-proximal 9-kD a fragment obtained by cleaving M I with formic acid. The binding was inhib ited by monoclonal antibodies (mAb) to region 46-70 of M1 and by an antiser um to region 21-45, whole as mAb to the middle and C-terminal regions had n o effect. Thus, the protease-binding domain was mapped to the N-terminal pa rt of M1.