S. Kato-emori et al., Cloning and characterization of the gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase in melon (Cucumis melo L. reticulatus), MOL GENET G, 265(1), 2001, pp. 135-142
We have isolated a cDNA for Cm-HMGR, encoding 3-hydroxy-3-methylglutaryl co
enzyme A (HMG-CoA) reductase in melon (Cucumis melo L. reticulatus: Genbank
Accession No. AB021862). Cm-HMGR encodes a polypeptide of 588 amino acids
that contains two transmembrane domains and a catalytic domain. Database se
arches revealed that Cm-HMGR shows homology to HMG1 (63.7%) and HMG2 (70.3%
) of tomato, to HMG1 (77.2%) and HMG2 (69.4%) of Arabidopsis thaliana, and
to HMGR of tobacco (72.6%). Functional expression in a HMG-CoA reductase-de
ficient mutant yeast showed that Cm-HMGR products mediate the synthesis of
mevalonate. Northern analysis revealed that the level of Cm-HMGR mRNA in th
e fruit increased after pollination and markedly decreased at the end of fr
uit enlargement. During ripening, Cm-HMGR mRNA levels increased markedly in
the fruit. In parallel with mRNA expression, Cm-HMGR activity increased af
ter pollination, whereas no Cm-HMGR activity was detectable during fruit ri
pening. Our results suggest that Cm-HMGR is important during early post-pol
lination development of the fruit in melon.