T. Tojima et E. Ito, A cyclic AMP-regulated negative feedforward system for neuritogenesis revealed in a neuroblastoma x glioma hybrid cell line, NEUROSCIENC, 104(2), 2001, pp. 583-591
We examined the role of second messengers during the neuritogenesis that ac
companies neuronal differentiation in a neuroblastoma x glioma hybrid cell
line (NG108-15). NG108-15 cells extended neurites after treatment with dibu
tyryl cyclic AMP. This dibutyryl cyclic AMP treatment evoked the synthesis
of voltage-dependent Ca2+ channel proteins in the cells. The number of neur
ites was decreased by Ca2+ influx under condition of high K+. Interestingly
, the increase of neurites stimulated by dibutyryl cyclic AMP and the decre
ase of neurites caused by high K+ were both reversible.
This is the first study to demonstrate that cyclic AMP regulates a negative
feedforward system for neuritogenesis, which links with Ca2+ signaling. Su
ch a dual role of cyclic AMP may play an important part in precise neurite
targeting. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights res
erved.